Cellular Quiescence - Methods and Protocols

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Springer US Cellular Quiescence - Methods and Protocols
Springer US - Cellular Quiescence - Methods and Protocols

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This detailed volume explores methods and protocols that aim to increase our understanding of how cells enter a quiescent state during homeostasis and how cells exit quiescence and re-enter differentiating cell divisions to restore damaged tissues, essential for developing new approaches in regenerative medicine in the future. The chapters in this book were designed to address cellular quiescence in prokaryote and eukaryote organisms, detection of quiescence (Hoechst/pyronin Y, FUCCI, CFSE, BrdU, H2B-GFP, CyTOF), quiescence in stem cells (skin, intestinal, neuronal, hematopoietic), genomic regulation (gene expression, transcription factors, lncRNA, RNA methylation), as well as analysis of the heterogeneity of quiescence by computer modeling. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Cellular Quiescence: Methods and Protocols offers a broad view of basic and cutting-edge technology to inspire research in this emerging field of cell biology.

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Anmerkung Illustrationen:
17 SW-Abb., 43 Farbabb., 44 Farbtabellen

1. Molecular Regulation of Cellular Quiescence: A Perspective from Adult Stem Cells and Its Niches

            Wai-Kin So and Tom H. Cheung


2. An In Vitro Model of Cellular Quiescence in Primary Human Dermal Fibroblasts

            Mithun Mitra, Linda D. Ho, and Hilary A. Coller


3. Flow Cytometric Detection of G0 in Live Cells by Hoechst 33342 and Pyronin Y Staining

            Ayad Eddaoudi, Stephanie Louise Canning, and Itaru Kato


4. Using Carboxy Fluorescein Succinimidyl Ester (CFSE) to Identify Quiescent Glioblastoma Stem-Like Cells

            Hassan Azari, Loic P. Deleyrolle, and Brent A. Reynolds


5. Isolation of Neural Stem and Progenitor Cells from the Adult Brain and Live Imaging of Their Cell Cycle with the FUCCI System

            Alexandra Chicheportiche, Martial Ruat, François D. Boussin, and Mathieu Daynac


6. Determination of Histone 2B-Green Fluorescent Protein (GFP) Retention in Intestinal Stem Cells

            Kevin R. Hughes and Yashwant R. Mahida


7. Detecting Hematopoietic Stem Cell Proliferation Using BrdU Incorporation

            Katie A. Matatall, Claudine S. Kadmon, and Katherine Y. King


8. Cell Cycle Analysis by Mass Cytometry

            Gregory K. Behbehani


9. Preparation and Analysis of Saccharomyces cerevisiae Quiescent Cells

            Marla M. Spain, Sarah G. Swygert, and Toshio Tsukiyama


10. Identifying Quiescent Stem Cells in Hair Follicles

            Christine N. Rodriguez and Hoang Nguyen


11. Single EDL Myofiber Isolation for Analyses of Quiescent and Activated Muscle Stem Cells

            Caroline E. Brun, Yu Xin Wang, and Michael A. Rudnicki


12. Investigating Cellular Quiescence of T Lymphocytes and Antigen-Induced Exit from Quiescence

            Kai Yang and Hongbo Chi


13. Retroviral Transduction of Quiescent Murine Hematopoietic Stem Cells

            Chun Shik Park and H. Daniel Lacorazza


14. Analysis of Murine Hematopoietic Stem Cell Proliferation During Inflammation

            Emilie Jalbert and Eric M. Pietras


15. A Facile, In Vitro 384-Well Plate System to Model Disseminated Tumor Cells in the Bone Marrow Microenvironment

            Johanna M. Buschhaus, Kathryn E. Luker, and Gary D. Luker


16. Distinguishing States of Arrest: Genome-Wide Descriptions of Cellular Quiescence Using ChIP-Seq and RNA-Seq Analysis

            Surabhi Srivastava, Hardik Gala, Rakesh K. Mishra, and Jyotsna Dhawan


17. Analysis of lncRNA-Protein Interactions by RNA-Protein Pull-Down Assay and RNA Immunoprecipitation (RIP)

            Holger Bierhoff


18. Analysis of MicroRNA-Mediated Translation Activation of In Vitro Transcribed Reporters in Quiescent Cells

            Syed I.A. Bukhari, Samuel S. Truesdell, and Shobha Vasudevan


19. Genome-Wide Identification of Transcription Factor Binding Sites in Quiescent Adult Neural Stem Cells

            Shradha Mukherjee and Jenny Hsieh


20. Study Quiescence Heterogeneity by Coupling Single-Cell Measurements and Computer Modeling

            Jungeun Sarah Kwon, Xia Wang, and Guang Yao

Lacorazza, H. Daniel;Lacorazza
Includes cutting-edge methods and protocols for the study of cellular quiescence

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Springer US
Softcover reprint of the original 1st ed. 2018


11. September 2018
0.254 x 0.178 x 0.018 m; 0.603 kg
€ 149,99
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