HIV Reservoirs - Methods and Protocols

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Springer US HIV Reservoirs - Methods and Protocols
Springer US - HIV Reservoirs - Methods and Protocols

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Beschreibung

This book details the development of methods and models to study the HIV-1 viral reservoir with the ultimate goal of achieving a functional cure of HIV infection. Chapters are divided into six parts covering cell lines, in vitro and ex vivo primary cell models of persistent infection, in vitro and ex vivo tissue-derived models, infected animal models human immune cells, methods of detection and analysis of the reservoir, and current approaches to achieve either a functional cure or cART-free long-term remission. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.


 


Authoritative and cutting-edge, HIV Reservoirs: Methods and Protocols provides a comprehensive, updated collection of state-of-art methodologies and models to tackle the HIV-1 viral reservoir.

Weitere Informationen

Anmerkung Illustrationen:
XIV, 451 p. 61 illus.
Inhaltsverzeichnis:

Part I: Cell lines with Minimal but Inducible Proviral Expression


1. Jurkat-derived (J-Lat, J1.1 and Jurkat E4) and CEM-derived T cell lines (8E5, ACH-2) as Models of Reversible Proviral Latency


Anthony RODARI, Guido Poli, and Carine VAN LINT



 


2. U1 and OM10.1. Myeloid Cell Lines as Surrogate Models Of Reversible Proviral Latency


Guido Poli


 


Part II: In vitro and ex vivo primary cell models of minimal but inducible proviral expression


3. An In Vitro System to Model the Establishment and Reactivation of HIV-1 Latency


in primary human CD4+ T cells


Rui Li and Fabio Romerio


 


4. The cultured TCM model of HIV latency


Alberto Bosque


 

5. A Reliable Primary Cell Model for HIV latency: The QUECEL (Quiescent Effector Cell Latency) Method

Meenakshi Shukla, Fredrick Kizito, Uri Mbonye, Kien Nguyen, Curtis Dobrowolski, and Jonathan Karn


 


6. TGF-b signaling supports HIV latency in a Memory CD4+ T cell based in vitro model


Sydney Bergstresser and Deanna A. Kulpa


 


7. Flow Cytometry Sorting of Memory CCR6+CD4+ T-Cells for HIV Reservoirs Quantification

Amélie Cattin, Augustine Fert, Delphine Planas, and Petronela Ancuta


 


8. Human monocyte-derived macrophages (MDM) - Model 1 (GM-CSF)


Claudia Alteri, Lorenzo Piermatteo, Francesca Ceccherini Silberstein, Valentina Svicher, and Carlo Federico Perno


 


9. Human monocyte-derived macrophages (MDM) - Model 2


Francesca Graziano, Elisa Vicenzi, and Guido Poli


 


10. Modeling HIV latency in astrocytes with the human neural progenitor cell line HNSC.100


Amelie Bauer


 


11. Hematopoietic stem and progenitor cells (HSPCs)


Valeri H. Terry, Gretchen E. Zimmerman, Maria C. Virgilio, Mark M. Painter, Dale Bixby, and Kathleen L. Collins


 


Part III: In vitro and ex vivo tissue-derived models of reversible proviral latency


12. HIV reservoirs: Modeling, Quantification and Approaches to a Cure


Louise A. Ouattara, Nikolas C. Vann, and Gustavo F. Doncel



 


13. More than a gender issue: Testis as a distinctive HIV reservoir and its implication for viral eradication


Jean-Pierre Routy, Franck P. Dupuy, John Lin, and Stéphane Isnard


 


Part IV: Animal Models


14. Experimental Models to Study HIV Latency Reversal from Male Genital Myeloid Cells


Fernando Real, Yonatan Ganor, and Morgane Bomsel


 


15. Decidua basalis: An ex vivo Model to Study HIV-1 Infection during Pregnancy and Beyond


Nabila Jabrane-Ferrat and Hicham El Costa


 


16. HIV Reservoirs: Modeling, Quantification, and Approaches to a Cure


Amir Dashti, Vidisha Singh, and Ann Chahroudi


 


17. Human Hematopoietic Stem Cell (HSC)-Engrafted NSG Mice for HIV Latency Research


Triana Rivera-Megias, Nhut M Le, and Alonso Heredia


 



18. Inducing long-term HIV-1 latency in the TKO-BLT mouse model


Yunyun Di and Kerry J. Lavender


 


Part V: Methods for detection and analysis of the reservoir



19. In situ multiplexing to identify, quantify, and phenotype the HIV-1/SIV reservoir within lymphoid tissue


Kathleen Busman-Sahay, Michael D. Nekorchuk, Carly Elizabeth Starke, Chi Ngai Chan, and Jacob D. Estes



 



20. Single-cell multiparametric analysis of rare HIV-infected cells identified by duplexed RNAflow-FISH


Mathieu Dubé and Daniel E. Kaufmann



 



21. Ex vivo differentiation of resting CD4+ T lymphocytes enhances detection of replication competent HIV-1 in viral outgrowth assays


Elizabeth R. Wonderlich, Monica D. Reece, and Deanna A. Kulpa


 


22. Quantitative Viral Outgrowth Assay to Measure the functional SIV Reservoir in Myeloid Cells


Abreu CM, Veenhuis RT, Shirk EN, Queen SE, Bullock BT, Mankowski JL, Gama L and Clements JE


 


23. Near-full-length single-genome HIV-1 DNA sequencing


Guinevere Q. Lee and Mathias Lichterfeld


 


24. TILDA: Tat/rev Induced Limiting Dilution Assay


Cynthia Lungu and Francesco A. Procopio


 


Part VI: Cure and long-term remission strategies


25. Latency Reversal and Clearance of Persistent HIV Infection


David M. Margolis


 


26. Cure and long-term remission strategies


Luisa Mori and Susana T. Valente


 


27. Pathways Toward a Functional HIV-1 Cure: Balancing Promise and Perils for CRISPR Therapy


Jonathan Herskovitz, Mahmudul Hasan, Milankumar Patel, Bhavesh D Kevadiya,and Howard E. Gendelman






Herausgeber:
Poli, Guido;Poli
Vicenzi, Elisa;Vicenzi
Romerio, Fabio;Romerio
Bemerkungen:
Includes cutting-edge methods and protocols


Provides step-by-step detail essential for reproducible results


Contains key notes and implementation advice from the experts

Medientyp:
Buch gebunden
Verlag:
Springer US
Sprache:
Englisch
Auflage:
1st ed. 2022
Seitenanzahl:
451

Stammdaten

Produkttyp:
Buch Gebunden
Veröffentlichungsdatum:
12. Dezember 2021
Verpackungsabmessungen:
0.254 x 0.178 x 0.025 m;
GTIN:
09781071618707
DUIN:
GJQDNSTV3P8
€ 149,99
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